Endonuclease IV and T4 ligase enhanced detection of mutations in low abundance
Analyst, 2024, Accepted ManuscriptDOI: 10.1039/D3AN02083E, CommunicationBo Li, Chunyan WangWe propose a mutant detection approach based on endonuclease IV, DNA ligase in combination with qPCR. Enzymes functioned cooperatively to facilitate PCR for low …